Ultrastructure of primary pacemaking cells in rabbit sino‐atrial node cells indicates limited sarcoplasmic reticulum content
نویسندگان
چکیده
منابع مشابه
Pacemaker Synchronization of Electrically Coupled Rabbit Sinoatrial Node Cells
The effects of intercellular coupling conductance on the activity of two electrically coupled isolated rabbit sinoatrial nodal cells were investigated. A computer-controlled version of the "coupling clamp" technique was used in which isolated sinoatrial nodal cells, not physically in contact with each other, were electrically coupled at various values of ohmic coupling conductance, mimicking th...
متن کاملUltrastructure of Sarcoplasmic Reticulum Preparations
Fragmented sarcoplasmic reticulum (FSR) from rabbit muscle was examined by positive staining, negative staining, and freeze-etch electron microscopic techniques in the absence and presence of calcium transport conditions. The existence of 30-40 A particles covering the outer surface of FSR vesicles was confirmed by two different negative stains in unfixed, glutaraldehyde-fixed and osmium tetrox...
متن کاملSpecialised pacemaking cells in the rabbit urethra.
1. Collagenase dispersal of strips of rabbit urethra yielded, in addition to normal spindle-shaped smooth muscle cells, a small proportion of branched cells which resembled the interstitial cells of Cajal dispersed from canine colon. These were clearly distinguishable from smooth muscle in their appearance under the phase-contrast microscope, their immunohistochemistry and their ultrastructure....
متن کاملDistribution of atrial and nodal cells within the rabbit sinoatrial node: models of sinoatrial transition.
BACKGROUND In the sinoatrial node (SAN) the course of the action potential gradually changes from the primary pacemaker region toward the atrium. It is not known whether this gradient results from different intrinsic characteristics of the nodal cells, from an increasing electrotonic interaction with the atrium, or from both. Therefore we have characterized the immunohistochemical, morphologica...
متن کاملSarcoplasmic reticulum Ca2+ pumping kinetics regulates timing of local Ca2+ releases and spontaneous beating rate of rabbit sinoatrial node pacemaker cells.
RATIONALE Sinoatrial node cells (SANCs) generate local, subsarcolemmal Ca(2+) releases (LCRs) from sarcoplasmic reticulum (SR) during late diastolic depolarization. LCRs activate an inward Na(+)-Ca(2+) exchange current (I(NCX)), which accelerates diastolic depolarization rate, prompting the next action potential (AP). The LCR period, ie, a delay between AP-induced Ca(2+) transient and LCR appea...
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ژورنال
عنوان ژورنال: FASEB BioAdvances
سال: 2020
ISSN: 2573-9832,2573-9832
DOI: 10.1096/fba.2018-00079